Charge reduction and neutralization of electro-sprayed peptides is realized by selective gas-phase photocleavage of tailored covalent tags. We demonstrate this here for the first time for a large polypeptide, i.e. insulin. This is an important step towards the controlled neutralization, ionization of proteins, which will be required in novel matter-wave beam splitters and interference detectors for proteins.
J. Schätti, M. Kriegleder, M. Debiossac, M. Kerschbaum,
P. Geyer, M. Mayor, M. Arndt, V. Köhler
Neutralization of Insulin by Photocleavage in High Vacuum
Chemical Communications 55, 12507-12510 (2019),
DOI: 10.1039/c9cc05712a.
